Adhesion of Platelets to Human Artery Subendothelium: Effect of Factor Vill-von Willebrand Factor of Various Multimeric Composition
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چکیده
The relationship between the multimeric size of factor VIII-von Willebrand factor (FVlll-vWF) and the support of platelet adhesion to subendothelium was studied in an annular perfusion chamber, employing human renal and umbilical arteries. Commercial factor VIII concentrates containing multimers of low molecular weight that had been shown not to correct the bleeding time upon infusion into patients with von Willebrand’s disease did not support platelet adhesion in the perfusion chamber. Cryoprecipitate and two experimental FVlll-vWF concentrates contaming muftimers of high molecular weight supported platelet adhesion. Factor Vlll-vWF purified from cryoprecipitate was subdivided into three fractions of different molecular weights (6.0-14.0. 4.0-9.0. and 3.07.5 x i05 dalton). These fractions appeared to bind equally
منابع مشابه
Adhesion of platelets to human artery subendothelium: effect of factor VIII-von Willebrand factor of various multimeric composition.
The relationship between the multimeric size of factor VIII-von Willebrand factor (FVIII-vWF) and the support of platelet adhesion to subendothelium was studied in an annular perfusion chamber, employing human renal and umbilical arteries. Commercial factor VIII concentrates containing multimers of low molecular weight that had been shown not to correct the bleeding time upon infusion into pati...
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The characteristics of the intact factor VIll/von Willebrand factor protein binding to human platelets was compared to 2-mercaptoethanol-treated factor VIll/von Willebrand factor protein and to fractions of plasma factor VIll/von Willebrand factor protein that elute after the void volume. These studies indicate that the factor VIlI/von Willebrand factor protein larger size oligomers bind prefer...
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We have studied the binding of von Willebrand factor to extracellular matrices of endothelial cells and to the vessel wall of human umbilical arteries in relation to its function in supporting platelet adhesion. CLB-RAg 201, an MAb against von Willebrand factor, completely inhibits the binding of von Willebrand factor to collagen type I and type III. CLB-RAg 201 does not inhibit the binding of ...
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BACKGROUND Thermal angioplasty methods heat the arterial wall. We related platelet adhesion to the temperature to which subendothelium and purified adhesive proteins had been exposed. METHODS AND RESULTS Cultured subendothelium, purified von Willebrand factor, collagen types I and III, or fibronectin was applied to glass coverslips. Coverslips were mounted on a heating device that applied a t...
متن کاملFactor VIII/von Willebrand factor in subendothelium mediates platelet adhesion.
The present studies were undertaken to determine whether factor VIII/von Willebrand factor (vWF) present in the vessel wall (in addition to that in plasma) may mediate the attachment of platelets to subendothelium. Subendothelium from everted rabbit aorta was exposed to human citrated blood flowing through an annular perfusion chamber at 40 mL/min (wall shear rate of 2,600 s-1 for five minutes)...
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